Isolation, Partial Purification and Immobilization of Neuraminidase enzyme from Vibrio cholera

Authors

  • Kumari C. K.
  • Diwedi K.
  • Singh S. K.
  • Shukla S.
  • Singh M.

Keywords:

Vibrio cholera, Silica gel, Acetone, Neuraminidase, SDS-PAGE, Sodium Alginate

Abstract

Vibrio cholera was isolated from pond water on TCBS media. Enzyme (Neuraminidase) was isolated from Vibrio cholera and concentration was quantified using Lowry’s method. The concentration is proportional to the absorbance. The difficult ranges of acetone were chosen for precipitation and enzyme quantification was performed for precipitated proteins obtained from their range. For acetone from 70% & 100% was chosen as from established sources it was found that 100% acetone give maximum concentration of enzyme. Enzyme sample obtained from precipitation with different acetone was then subjected to SDS-PAGE analysis. The band pattern of enzyme was observed on the basis of their molecular weight and purity after SDS-PAGE. The molecular weight of Neuraminidase was found to 50 kd. The purified enzyme was the subjected to immobilization with in sodium alginate beads.

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Published

2020-08-19

How to Cite

Kumari C. K., Diwedi K., Singh S. K., Shukla S. and Singh M. (2020) “Isolation, Partial Purification and Immobilization of Neuraminidase enzyme from Vibrio cholera”, International Journal of Research in BioSciences (IJRBS), 3(2), pp. 53-57. Available at: http://ijrbs.in/index.php/ijrbs/article/view/114 (Accessed: 22December2024).

Issue

Vol. 3 No. 2 (2014): April

Section

Articles