Specific detection of human pathogenic Vibrio species in water bodies of north Indian region by using multiplex polymerase chain reaction

Abstract

The purpose of this study was to investigate the prevalence of Human pathogenic Vibrio species in water bodies of north Indian region using the traditional culture and multiplex PCR methods. Vibrio species are very much prevalent in water bodies of tropical region.The study was conducted on 30 fresh water bodies’ samples collected from rivers and ponds of north Indian zones. For this species specific primers were designed targeting the tox gene of the five pathogenic species specific and confirmation was done using multiplex PCR technique for rapid detection of the five selected pathogenic species including V. cholerae, V. alginolyticus, V. parahaemolyticus, V. mimicus and V. vulnificus. Out of the five targeted species three were present in the samples. The major rivers of north India shows high rate of contamination and Vibrio cholera was most abundant among all.