Production of collagenase by Bacillus KM369985 isolated from leather sample

Abstract

Collagen is a major fibrous element of skin, bones, tendons, cartilage, blood vessels and teeth found in all multicellular organisms and which is also the most representative protein of leather wastes. Collagenases are endopeptidase that can hydrolyze both native and denatured collagens. Collagen peptides, the product of collagen degradation, possess various biological activities of industrial and medical interest such as ingredient in drugs, drinks, food, cosmetics etc. In present investigation, twenty six collagenase producing bacteria were isolated from fifty leather samples, one of most efficient collagenase producers was isolated, identified and was found to be belonging to genus Bacillus. 16s r RNA sequencing was done and the sequence was deposited to GenBank with accession number Bacillus KM 369985. The isolate was able to produce 600U/ml in 72h. The optimum temperature, substrate and pH were 37°C, 1.5% and7.5 respectively. Effect of incubation period and inoculum percentage was also studied. The collagenase production was strongly inhibited by Hg+2, EDTA and β-mercaptoethanol however Fe2+, Zn2+and DMSO enhanced its production. Isolate was capable of hydrolyzing other protein substrates such casein, gelatin, and keratin. Hence this isolate can be efficiently used to treat tannery waste and recycling of these organic materials.