Effect of supplementation of Entada phaseoloides seed powder on growth performance, carcass characteristics and haemato- biochemical parameters along with its effect on expression of HSP70 in broiler chicken

Abstract

A study was conducted to evaluate E. phaseoloides seed powder as a dietary supplementation to study the growth performance, carcass characteristics, haemato-biochemical changes along with its effect on expression of HSP70 in broiler chicks. A total of 90 numbers of one day old chicks were randomly distributed into 3 groups of 3 replicates having 10 birds each. The dietary treatment comprised of a basal diet as control, basal diet supplemented with 0.5% E. phaseoloides seed powder level as T1 and basal diet supplemented with 1% E. phaseoloides seed powder level as T2 group. Body weight, feed consumption were recorded weekly and accordingly FCR (Feed conversion ratio) were calculated. At the end of 5th week, blood was collected from 3 birds from each replicate for haematobiochemical parameters and then sacrificed to determine the carcass characteristics. Supplementation of E. Phaseoloides seed powder significantly improved the live weight (P<0.05) and FCR, which was statistically comparable to control and T1 (0.5%) group. Dietary treatment did not influence the carcass characteristics significantly. Mean values of serum protein, albumin, globulin, A/G ratio, glucose, cholesterol, PCV, ESR, Hemoglobin, SGOT and SGOT were similar among the groups except for significant variation (P<0.001) seen in serum protein and Hb. Gene expression study in spleen and liver tissue showed significant (P<0.05) down regulation of hsp70 gene in T2 group. Further confirmation by immunoblotting study of HSP70 protein also showed significant decrease in the protein level in liver and spleen of treatment group. Although, expression of the hsp70 mRNA and the HSP70 protein was evident in heart tissue, However it was not significant (P>0.05). From the result of present study it can be inferred that dietary inclusion of 1% E. phaseoloides seed powder can promote growth performance in broilers without any side-effects.