Initiation and establishment of callus for in vitro studies in M. emerginata

Abstract

In present study unorganized tissues of M. emerginata were established on MS medium to determine the most suitable conditions for production of maximum biomass in cultures. Leaves shoot apices and flowers of M. emerginata used as explants showed poor response towards callus formation, but germinating seeds were used as explants for initiation and establishment of callus. Tissues were harvested at the age of 2, 4, 6, 8, 10 and 12 weeks and their growth indices were calculated on fresh weight basis. Best callusing was obtained on MS medium supplemented with 1.5 mg/L BAP + 1 mg/L NAA in M. emerginata, maximum GI was calculated at eight week callus (3.24).Main objective was to identify, isolate and estimate primary as well as medicinally and economically important secondary metabolites qualitatively and quantitatively to compare with in vivo studies.