Application of new microbial plant resistance/plant growth protection inducers for increasing Chinese cabbage plant tolerance against parasitic nematode Heterodera schachtii Schmidt

Abstract

Impact of new Microbial Plant Resistance/Plant Growth Protection (PR/PGP) Inducers for increasing tolerance of Brassica rapa subsp. pekinensis (Chinese cabbage) to parasitic nematode Heterodera schachtii Schmidt was studied. In the greenhouse conditions on the artificial invasive nematode background the accumulation of Chinese cabbage biomass was increased as follows: under impact of Avercom nova-2 - up to 94%, Phytovit - up to 55%, Violar - up to 32% and Avercom - up to 17%, accordingly as compared to control. In the laboratory conditions the biomass of affected by nematode and treated by microbial PR/PGP inducers plants was increased up to 26-43 % as compared to biomass of control affected by nematode plants. Influence of Microbial PR/PGP Inducers on molecular-genetic indexes of Chinese cabbage resistance to nematode Heterodera schachtii was investigated. The increase in the difference of Dot-blot hybridization index up to 27-49% between cytoplasmic mRNA and small regulatory RNA (si/miRNA), isolated from control and affected by nematode and treated by microbial PR/PGP inducers plants was shown. Silencing activity of si/miRNA isolated from affected by nematode and treated by microbial PR/PGP inducers plants was increased up to 32-67 % in the wheat embryo cell free system. Obtained data testify that microbial PR/PGP inducers stimulate synthesis in the plant cells of immune-protective si/miRNA. As a result of this, plant tolerance against this parasitic nematode is increased.