High performance thin layer chromatography pattern of Excoecaria agallocha (Thillai mangrove)

Abstract

An investigation of phytochemical profile and HPTLC pattern of aromatic ethno-medicinally important methanol extracts of leaf, stem and root of Thillai mangrove Excoecaria agallocha, is present here. Qualitative phytochemical screening was done by standard biochemical methods. Standard serial comparative methodological HPTLC experiments (2 µl, 5 µl, 10 µl and 20 µl of 500mg/ml methanol extracts samples of leaf, stem and root) were performed along with various parameter optimization. 10 µl volumes were used as a standard for final interpretation and various profiles were compared [scanned under UV – 254 nm, UV – 366 nm (before and after derivatization) and under visible light (after derivatization)] and specific, reproducible, and faithful HPTLC fingerprints scenario of phytochemicals were achieved. This study may become potent phytochemical- statement for analysis phytochemicals as visualized graphical document as well as physical separation – detection that canexamine the unique authenticity of Excoecaria agallocha species and qualitative as well as further quantitative understanding of its phyto-compounds.