Decolorization, degradation and azo-reductase study by bacterial transformation of reactive red HE8b

Abstract

To achieve this goal (remediation of dyes) decolorization and degradation was proved by our identified culture DN1 by performing various process parameter. Among them optimized results was dye concentration 200mg.L-1(86.2%), temperature 400C (91.5%), inoculum size 2.5 mL (92.0%) and pH 6.5 (89.5%). Various types of carbon and nitrogen sources are supplemented into medium to enhance the decolorization. Among them lactose (0.6%) (94.1%) and yeast extract (0.5g%) (96.5%) was optimum as carbon and nitrogen source respectively. Furthermore, degradation was also proved by performing UV-Vis, HPTLC and FTIR analysis. Azo-reductase study was also carried out from DN1. Total protein determination was performed for crude and partially purified enzyme, followed by protein determination activity of Azo-reductase was checked in both acetate buffer (30.68±0.05) and phosphate buffer (12.64±0.09). Acetate buffer gave higher activity rather than phosphate buffer, so characterization was performed from acetate buffer. Among them methyl red was used as a fixed substrate, optimum pH was 5.5 and optimized temperature was.