Isolation and screening of hydrocarbon degrading bacteria from soil near Kadi (Gujarat) region

Abstract

Ten bacterial isolates were recovered from the Hydrocarbons contaminated soil samples from an ONGC well and petrol pump near Kadi region, Gujarat. These organisms showed optimum growth in presence of hydrocarbons like crude oil, Diesel, Kerosene and 2T oil. The Hydrocarbons degrading isolates were identified as species of genera Acinetobacter, Micrococcus, Methylobacterium, Pseudomonas sp, Rhodococcus and Noccardia. All the ten isolates degraded 1% Hydrocarbons (Petrol, Diesel, Kerosene and 2T oil) in Bushnell Haas broth. Highest biodegradation of Hydrocarbon was found to be 70% by the isolate KD1 which belonged to genus Pseudomonas sp. Other isolates showed biodegradation in the range of 30%-55%. On addition of 1% Tween 80 biodegradation reduced in majority of cases.