Impact of different growth regulators supplemented in MS medium on induction of callus from leaf explants of natural sweetener, Stevia rebaudiana (Bertoni)

Abstract

Stevia rebaudiana (Bertoni) is commonly known as natural plant sweetener or sweet herb was taken for tissue culture study. From the healthy plant, cultivated in the local garden fresh leaves were collected and inoculated in MS medium supplemented with 3% sucrose and six different concentration of 2,4-D and three different concentrations of BAP and kinetin, either alone 2,4-D or with BAP or with kinetin. The growth rate, colour and texture and percentage of response of call using were recorded and the mean of the data were tabulated in the table. From the table in may be noted that MS + 2.0 mg/l 2,4-D alone induced callusing in 68.00 %, while MS + 2.0 mg/l 2,4-D + 0.5 mg/l BAP induced callusing in 52 % of the explants. 78.15 % of response with respect to callus induction was observed in MS + 2.0 mg/l 2, 4-D + 1.0 mg/l KN which was followed by 70 % in MS + 2.0 mg/l 2,4-D + 0.5 mg/l KN. Similarly higher growth rate was also noted in the same concentration where higher percentage of response was found. In this culture conditions calli were green and compact in the texture. These calli were sub culture in the same medium aften 21st days.