Bioefficacy of leaf extracts of Clerodendrum infortunatum L. and Eupatorium odoratum L. on both quantitative and qualitative analysis of midgut protein of sixth instar larvae of Orthaga exvinacea Hampson (Lepidoptera: Pyralidae)

Abstract

The impact of methanolic leaf extract of Clerodendrum infortunatum and Eupatorium odoratum on protein concentration and protein profile in the midgut tissue of sixth instar larvae of Orthaga exvinacea were studied under laboratory conditions. The different concentrations (1%, 2%, 3%, 4%, and 5%) of each botanical treated mango leaves were fed to the sixth instar larvae. After 48 hours larvae were sacrificed to collect midgut tissue and analysis were done. The results of quantitative and qualitative estimation of protein content in the midgut tissue of both botanicals treated and control showed that there was some considerable decrease in the amount of protein and alterations in protein profile were observed in treated tissue compared to control. The decrease in level of protein concentration was correlated with the increase in concentration of botanicals. Formation of new protein bands and disappearance of some protein bands were noticed in protein profile of treated tissues with increase in botanical concentrations. Among the botanicals tested E. odoratum was more effective than C. infortunatum and this reveals the potency of both botanicals to be used as natural biopesticides.