Effect of non thermal pulsed atmospheric pressure plasma jet on transcription of malignant associated genes

Abstract

One of challenges is the application of non thermal plasms directly on the surface of human body or on internal organs, tissues and genes, and the ability of therapeutic applications in malignancy. The effect of cold plasma are due to charged particles, reactive nitrogen, UV, photons and intense electric field. For obtaining efficient action on all types of cells generally to reach benefits for cancer cell therapy. Researchers have found that certain types of tumors have proteins on their surface that are specific to the tumors and not found anywhere else in the body. This is key to the type of cancer treatment involved. In our study we used pulsed atmospheric – pressure plasma jet (PAPPJ), The study carried out through four groups of mice, one group is normal mice, and three groups are solid Ehirlich Tumor transplanted mice at thigh region, one of which was non exposed, the second was exposed to 15 pulse of plasma and the third exposed to 30 pulse of plasma. The exposure extended for 4 weeks, twice weekly. Using RNA isolation and cDNA synthesis, the study demonstrated that: firstly, plasma jet exposure reduce the rate of tumor growth without completely treatment, and the reduction of tumor growth rate increase as the result of pulse number increase, secondly, plasma jet induce and affect the amplitude of gene transcription, which may be the genes associated with malignancy (up regulated and down regulated genes are major for treated groups). Non Thermal plasma jet up regulated some types of genes and down regulated another types of genes, these genes may be includes, apoptotic genes, tumor suppressor genes and oncogenes. And because of the detection of the level of transcription was generally and non specific through using arbitrary primers, therefore, we recommend to carry out more specific studies for recognizing the genes which were up regulated and the others which were down regulated as the result of plasma exposure.